PRACTICAL ASPECTS OF ONE-DIMENSIONAL NMR 2023-24

PROGRAM

Teacher: Dr. Ileana Menegazzo (DiSC)

Theory (slides in English, lesson in Italian – about 2h)

Date: 20/02/2024, at 10:00

Location: aula N (DiSC)

Overview of the NMR spectrometers in the Department, with relative probes and software.

Safety rules in presence of magnetic fields (on line course is compulsory before the practical training).

Basic principles of the one-dimensional NMR experiment.

Preliminary operations: Editing Temperature - Lock - Shimming - Wobb

Acquisition and elaboration of the ¹H spectrum:

-        Acquisition parameters

-        Phase correction, baseline correction, calibration, integration

-        Field inhomogeneities: effects on the spectrum

-        Effects of the number of acquired and processed points, AQ time and window functions

-        RG and receiver saturation

-        D1: effect on the integration

-        90° pulse calibration

The quick T1 estimation

Acquisition and elaboration of the ¹³C spectrum:

-        ¹³C not decoupled and ¹³C {¹H} decoupled

-        DEPT

Solvent signal suppression with presaturation

Practical training at the Bruker AVANCE III 400 NMR - Department of Chemical Sciences (about 4h)

Date: to be defined

Location: DiSC (NMR facilities)

Acquisition and processing of the experiments in groups.

Non-Italian students will be grouped together and the practical training will be in English.